Anti-AP2M1 antibody [EP2695Y] ab75995 from Abcam

Product Specs
ItemAnti-AP2M1 antibody [EP2695Y]
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab75995
Antibody TypePrimary
ApplicationsWestern blot
ClonalityMonoclonal
Conjugate/TagUnconjugated
HostRabbit
Quantity10 UL, 40 UL, 100 UL
CloneEP2695Y
IsotypeIgG
Gene NameAP2M1
ReactivityHuman, Mouse, Rat
TargetAP2M1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Autophagy-mediated NKG2D internalization impairs NK cell function and exacerbates radiation pneumonitisFrontiers in ImmunologyNovember 24, 2023Ruiqing Wang, Xinyue Ma, Xinyu Zhang, Dizhi Jiang, Hongyuan Mao, Zerun Li, Yu Tian, Bo Chengantibodies as follows: GAPDH (#ab9485, Abcam), β-tublin (#ab179511, Abcam), AP2A1 (#ab189995, Abcam), AP2M1(#ab75995, Abcam), LAMP2 (#ab13524, Abcam), LC3 (#ab62721, Abcam), NKG2D (#ab36136, Abcam), CXCR3 (#ab288437,

(2) Kinetic investigation reveals an HIV-1 Nef-dependent increase in AP-2 recruitment and productivity at endocytic sitesbioRxivApril 25, 2023Yuichiro Iwamoto, Anna Ye, Cyna Shirazinejad, James H. Hurley, David G. Drubinanti-GAPDH (1:10000 in TBST, RT 1 hr, ab9485), anti-AP-2M1 (1:500 in 0.5% milk TBST, 4°C o/n, Abcam, ab75995), and anti-Tag(CGY)FP (1:2000 in 0.5% milk TBST, 4°C o/n, Evrogen AB121). Blots were then incubated

(3) SARS-CoV-2 down-regulates ACE2 through lysosomal degradationMolecular biology of the cellDecember 1, 2022Lu Y, Zhu Q, Fox DM, Gao C, Stanley SA, Luo K.(Santa Cruz; sc-18822, H4B4); anti-clathrin heavy chain (Santa Cruz; sc-12734, TD.1); anti-μ2 (Abcam; ab75995, EP2695Y); anti–caveolin-1 (Cell Signaling Technology; 3267T, D46G3); anti-Strep (IBA; 2-1509-001);

(4) Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cellbioRxivSeptember 6, 2022Maritza Puray-Chavez et al.#A5316, 1:5000), Vinculin (Santa Cruz #sc-73614, 1:2000), AAK1 (Bethyl #A302–146A, 1:1000), AP2M1 (Abcam #ab75995, 1:1000), pAP2M1-T156 (Cell Signaling #3843, 1:1000), SARS-CoV-2-N (Sino Biological #40588-T62, 1:500),

(5) Targeting Adiponectin Receptor 1 Phosphorylation Against Ischemic Heart FailureCirculation ResearchJuly 8, 2022Di Zhu, Zhen Zhang, Jianli Zhao, Demin Liu, Lu Gan, Wayne Bond Lau, Dina Xie, Zhijun Meng, Peng Yao, Jumpei Tsukuda, et al.,Cell Signaling Technology,7399,1:1000,,https://www.cellsignal.com/product/productDetail.jsp?productId=7399 Anti-AP2M1 rabbit monoclonal antibody,Abcam,ab75995,1:2000,,https://www.abcam.com/ap2m1-antibody-ep2695y-ab75995.html Anti-GRK2 rabbit polyclonal antibody,Cell Signaling Technology

(6) Induced nanoscale membrane curvature bypasses the essential endocytic function of clathrinJournal of Cell BiologyJuly 4, 2022Robert C. Cail, Cyna R. Shirazinejad, David G. Drubinheavy chain antibody (ab21679, rabbit; Abcam) at 1:500 dilution in TBSt with 5% milk, AP2M1 antibody (ab75995, rabbit; Abcam) at 1:1,000 dilution in TBSt with 5% milk, or FCHo2 antibody (ab122407, rabbit; Abcam)

(7) Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cellCell reportsJuly 13, 2021Puray-Chavez M, LaPak KM, Schrank TP, Elliott JL, Bhatt DP, Agajanian MJ, Jasuja R, Lawson DQ, Davis K, Rothlauf PW, et al.#A5316, 1:5000), Vinculin (Santa Cruz #sc-73614, 1:2000), AAK1 (Bethyl #A302-146A, 1:1000), AP2M1 (Abcam #ab75995, 1:1000), pAP2M1-T156 (Cell Signaling #3843, 1:1000), SARS-CoV-2-N (Sino Biological #40588-T62, 1:500),

(8) BIKE regulates dengue virus infection and is a cellular target for broad-spectrum antiviralsAntiviral ResearchDecember 1, 2020Szuyuan Pu et al.and blotted with anti-BIKE (Santa Cruz biotechnology, catalog sc-134284), anti-AP2M1 (Abcam, catalog ab75995), anti-GLuc (New England BioLabs, catalog E8023S), anti–phospho-AP2M1 (T156) (Cell Signaling, catalog

(9) Frustration of endocytosis potentiates compression-induced receptor signalingJournal of cell scienceSeptember 1, 2020Baschieri F, Le Devedec D, Tettarasar S, Elkhatib N, Montagnac G.BD Biosciences. Mouse monoclonal anti-αadaptin (cat. no. ab2807), rabbit monoclonal anti-AP2M1 (cat. no. ab75995) and rabbit monoclonal anti-EGFR (cat. no. ab2430) were purchased from Abcam. Rabbit polyclonal anti-α-adaptin

(10) Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral targetScience advancesAugust 1, 2020Yuan S, Chu H, Huang J, Zhao X, Ye ZW, Lai PM, Wen L, Cai JP, Mo Y, Cao J, et al.nuclear, ER, and cell membrane staining, respectively. Primary antibodies against human AP2M1 (Abcam, ab75995), anti–β-actin (Abcam, ab8227), anti–influenza NP (Abcam, ab104870), anti–ZIKV NS3 (GeneTex, GTX133309),

(11) Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosisNeurobiology of diseaseJuly 1, 2020Heaton GR, Landeck N, Mamais A, Nalls MA, Nixon-Abell J, Kumaran R, Beilina A, Pellegrini L, Li Y, International Parkinson Disease Genomics Consortium (IPDGC)., et al.1:2000), Mouse monoclonal to Alpha Adaptin (Thermo #MA1–064, 1:1000), Rabbit monoclonal to AP2M1 (Abcam #ab75995, 1:2000), Rabbit monoclonal to AP2S1 (Abcam #ab128950, 1:2000), Rabbit monoclonal to AP2B1 (Abcam #ab129168,

(12) Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 PhosphorylationDevelopmental cellAugust 19, 2019Wrobel AG, Kadlecova Z, Kamenicky J, Yang JC, Herrmann T, Kelly BT, McCoy AJ, Evans PR, Martin S, Müller S, et al.overnight at 4°C. The immune complexes were analyzed by Western blot with rabbit anti-AP2- μ2 (Abcam, (ab75995)), anti-AP2α (#AC1-M11, Pierce), Anti AP2-μ2 pT156 D4F3 (Cell Signalling), SNX9 (Sigma, HPA031410),

(13) USP9X Deubiquitylates DVL2 to Regulate WNT Pathway SpecificationCell reportsJuly 23, 2019Nielsen CP, Jernigan KK, Diggins NL, Webb DJ, MacGurn JA.(AP2A1) AC1-M11,Thermo Fisher,Cat #: MA3–061; RRID:AB_2056321 Anti-AP2M1 antibody [EP2695Y],Abcam,ab75995; RRID:AB_1309955 Anti-VANGL1,Sigma,Product #: HPA025235; RRID:AB_1858718 Cofilin (D3F9) XP Rabbit mAb

(14) WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback LoopCell reportsJanuary 2, 2019Agajanian MJ, Walker MP, Axtman AD, Ruela-de-Sousa RR, Serafin DS, Rabinowitz AD, Graham DM, Ryan MB, Tamir T, Nakamichi Y, et al.[EPR4700],Abcam,Cat# ab109397; RRID:AB_10866362 Rabbit monoclonal anti-AP2M1 [EP2695Y],Abcam,Cat# ab75995; RRID:AB_1309955 Mouse monoclonal anti-CTNNB1,BD Biosciences,Cat#163510 Mouse monoclonal anti-GSK-3

(15) Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteinsScientific reportsOctober 4, 2018Richter KN, Wildhagen H, Helm MS, Ußling JE, Schikorski T, Rizzoli SO.(SEM),3736.4,207.63,Synaptic Systems,155 003,rabbit polyclonal,1:500 AP2,19.136,1.546 (SEM),2324.7,81.99,Abcam,ab75995,rabbit monoclonal,1:500 APP,14.186,2.776 (SEM),6283.6,584.51,Millipore,MAB348,mouse monoclonal,1:100 Arc,18.419,0.545

(16) Anticancer kinase inhibitors impair intracellular viral trafficking and exert broad-spectrum antiviral effectsJournal of Clinical InvestigationApril 3, 2017Elena Bekerman et al.Blots were blocked and blotted with anti-AP1M1 (Abcam, catalog ab111135), anti-AP2M1 (Abcam, catalog ab75995), anti-GLuc (New England BioLabs, catalog E8023S), anti–phosphoAP2M1 (T156) (Cell Signaling, catalog

(17) Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial CellsPloS oneJanuary 1, 2016Chiang CF, Flint M, Lin JS, Spiropoulou CF.ANDV Gc (1:2000 dilution; catalog no. H1808-60, US Biological, Swampscott, MA, USA), AP2M1 (1:1000; ab75995, Abcam, Cambridge, MA, USA), ARF6 (1:200; sc-7971, Santa Cruz Biotechnology, Santa Cruz, CA, USA), caveolin-1

(18) Lysosome size, motility and stress response regulated by fronto-temporal dementia modifier TMEM106BMolecular and cellular neurosciencesJuly 1, 2014Stagi M, Klein ZA, Gould TJ, Bewersdorf J, Strittmatter SM.rabbit anti-AP2M1 at 1:500 (a kind gift of Pietro De Camilli, Yale University, equivalent to Abcam, ab75995). All antibodies were diluted in blocking buffer, and membranes were incubated overnight at 4°C. Following

(19) Role of AP1 and Gadkin in the traffic of secretory endo-lysosomesMolecular Biology of the CellJune 15, 2011Karine Laulagnier, Nicole L. Schieber, Tanja Maritzen, Volker Haucke, Robert G. Parton, Jean Gruenberg

(20) Mechanisms of single-stranded phosphorothioate modified antisense oligonucleotide accumulation in hepatocytesNucleic acids researchJune 1, 2011Koller E, Vincent TM, Chappell A, De S, Manoharan M, Bennett CF.

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Anti-AP2M1 antibody [EP2695Y] from Abcam

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Anti-AP2M1 antibody [EP2695Y] from Abcam

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